A wheel image acquisition assembly includes a base, a motor assembly and a camera module. One end of the base is for being connected to a support body in a wheel positioning apparatus, and the other end of the base is for being connected to the camera module. The motor assembly is mounted in the base and includes a motor for driving the camera module to rotate around the horizontal axis so as to adjust a pitch angle of the camera module. The camera module is used for acquiring image of wheels so as to determine the position of the wheels relative to a vehicle. The wheel image acquisition assembly can drive the camera module to rotate by means of the motor, so as to adjust the visual field range of the camera module, which is more flexible, and facilitates wheel alignment positioning in a complex environment.