A cell culture apparatus is provided. A cell culture apparatus according to an exemplary embodiment of the present invention comprises: a housing having an internal space which is filled with a culture medium for cell culture; and a support assembly which is provided in a plate form having a predetermined area, comprises a plurality of supports provided in multiple stages at a certain distance apart from each other in one direction, and is inserted in the internal space in the state where the plurality of supports are coupled to each other via at least one fastening bar.