The present invention belongs to the technical field of gloves, and relates to a biodegradable glove and a preparation method thereof. It comprises the following steps: S1, passing a glass fiber or steel yarn metal through a yarn tension controller and the spindle hole as the yarn core, and winding and wrapping the biodegradable filament yarn as the outer yarn to form the coated yarn for gloves, then weaving into textile gloves on a glove knitting machine; S2. gum dipping the textile gloves to form the degradable gloves. The preparation method is simple and easy to operate, and is suitable for large-scale production.