A method of treating a harvested organ or tissue for preservation for implantation into a patient has the steps of, harvesting an organ or tissue from a donor; placing the harvested organ or tissue into a container; filling the container with a fluid for preservation; sealing the container once filled; directing one or more sound wave treatments into the container to destroy bacteria or molds or fungi or virus and to stimulate the organ or tissue; and storing the container at a hypothermic temperature of about 4 degrees C. for storage prior to implantation.