发明公开
- 专利标题： ARTIFICIAL AXON BUNDLE
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申请号： US18034645申请日： 2021-10-28
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公开(公告)号： US20230390459A1公开(公告)日： 2023-12-07
- 发明人: Shinsuke SHIBATA , Hiroo KIMURA , Takayuki NISHIJIMA , Tomoko SHINDO , Hideyuki OKANO , Jiro KAWADA , Sayaka TOITA
- 申请人： KEIO UNIVERSITY , Jiksak Bioengineering Inc.
- 申请人地址： JP Tokyo
- 专利权人： KEIO UNIVERSITY,Jiksak Bioengineering Inc.
- 当前专利权人： KEIO UNIVERSITY,Jiksak Bioengineering Inc.
- 当前专利权人地址： JP Tokyo; JP Kawasaki-shi, Kanagawa
- 优先权： JP 20181354 2020.10.29
- 国际申请： PCT/JP2021/039869 2021.10.28
- 进入国家日期： 2023-04-28
- 主分类号： A61L27/38
- IPC分类号： A61L27/38
摘要：
The present invention relates to an axon bundle obtained by a method of culturing a nerve cell aggregation having one spheroid and an axon bundle extending from the spheroid
wherein the nerve cell aggregation consisting of a plurality of neurons having a cell body and an axon obtained by a method comprising the following step:
supplying a culture medium to one first chamber, one second chamber and one channel having the length of at least 1 mm, the width of 100 to 150 μm and the height of 100 to 200 μm, which connecting said first chamber and second chamber, wherein said first chamber, second chamber, and channel are contained in one of the modules disposed in a culture plate,
seeding the first chamber with a nerve cell derived from a stem cell or a spheroid of primary cultured nerve cells; and
culturing said nerve cell, thereby growing an axon bundle from said spheroid and extending them into said channel,
wherein the length of the axon bundle obtained is 1 mm or more, and the diameter of the axon bundles is 50 μm or more.
wherein the nerve cell aggregation consisting of a plurality of neurons having a cell body and an axon obtained by a method comprising the following step:
supplying a culture medium to one first chamber, one second chamber and one channel having the length of at least 1 mm, the width of 100 to 150 μm and the height of 100 to 200 μm, which connecting said first chamber and second chamber, wherein said first chamber, second chamber, and channel are contained in one of the modules disposed in a culture plate,
seeding the first chamber with a nerve cell derived from a stem cell or a spheroid of primary cultured nerve cells; and
culturing said nerve cell, thereby growing an axon bundle from said spheroid and extending them into said channel,
wherein the length of the axon bundle obtained is 1 mm or more, and the diameter of the axon bundles is 50 μm or more.
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