A display substrate has blocking structures. The display substrate includes a base substrate, a display structure on a display area of the base substrate, at least one blocking structure on a peripheral area around the display area of the base substrate, and a first water-blocking layer on the substrate. The first water-blocking layer may cover the display structure and the at least one blocking structure. The blocking structure may be configured to block cracks generated on the first water-blocking layer at a side of the blocking structure opposite from the display area from propagating to the display area.