A display substrate includes a plurality of first subpixels, a plurality of second subpixels and a plurality of third subpixels. In a first direction, the first subpixels and the third subpixels are arranged alternately to form a plurality of first subpixel rows, the second subpixels are arranged to form a plurality of second subpixel rows, and the first subpixel rows and the second subpixel rows are arranged alternately in a second direction. Lines connecting centers of two first subpixels and two third subpixels in two adjacent rows and columns form a first virtual quadrilateral, the two first/third subpixels are arranged at two/the other two opposite vertices of the first virtual quadrilateral respectively, and a corresponding second subpixel is located within the first virtual quadrilateral. Lines connecting centers of two second subpixels, one first subpixel and one third subpixel in adjacent three rows and columns form a virtual parallelogram.