The present disclosure provides a chip-on-film, a display substrate, a display device and a driving method. The display substrate includes a plurality of scanning lines and a plurality of cascaded shift register units coupled to the scanning lines. The display substrate further includes an output control signal line electrically coupled to each shift register unit for providing an enable signal for the shift register unit. The output control signal lines include at least two output control signal lines for providing different enable signals, and two adjacent cascaded shift register units are coupled to different output control signal lines.