An excreta sample capture device includes a primary influent pipe that receives influent from a toilet(s) and includes an upstream end and a downstream end. The excreta sample capture device also includes a first valve positioned at the downstream end of the primary influent pipe, and a second valve positioned between the upstream end and the downstream end of the primary influent pipe. In some cases, an excreta sample capture device further includes spray jet positioned above the second valve and a sample extraction vessel located downstream from the second valve. A method of using an excreta sample capture device includes receiving influent in the primary influent pipe, initiating a closing of the first valve, opening the second valve, and capturing a sample of the excreta.