A display substrate includes a substrate and a display region, an isolation region, a peripheral region and a hole disposed on the substrate, wherein the display region surrounds the hole, the isolation region is disposed between the display region and the peripheral region, and the peripheral region surrounds and adjoins the hole; the isolation region is provided with an isolation column and a first inorganic structure, the isolation column is disposed at a side of the substrate, and the first inorganic structure is disposed at a side of the isolation column away from the substrate; and the peripheral region is provided with a structure layer, and the structure layer is disposed on the substrate and includes a first organic structure and a second inorganic structure disposed along a direction perpendicular to the substrate; wherein the first inorganic structure, the second inorganic structure and the first organic structure all are made of an insulation material, and the first inorganic structure and the second inorganic structure are configured to encapsulate the display substrate.