A display substrate, a color filter substrate, a display panel and a display device are disclosed in the present application, relating to the field of display technologies. In the display substrate, because the area of the orthographic projection of the first opening region on the first base substrate is less, the area of the orthographic projection of the second opening region on the first base substrate is larger, and the area of the orthographic projection of the third opening region on the first base substrate is larger, the first subpixel of the display substrate emits less light, and the second subpixel and the third subpixel emit more light. In addition, because the wavelength of the first color is larger and the proportion of the light of the first color in the light emitted from the display substrate is less, the color cast of the display panel can be avoided and the display panel has a better display effect.