An organic light-emitting diode (OLED) display substrate, a manufacturing method thereof, and a display device are disclosed. In the OLED display substrate, an anode pattern is located at a side of an insulating layer away from a base substrate and is located in an effective display region; an organic light-emitting layer is located on the anode pattern; a periphery region includes a lead wire region and a virtual region that are at least partly overlapped; a lead wire is located in the lead wire region; the virtual region is provided with a virtual anode pattern, the virtual anode pattern is insulated from the lead wire; the periphery region further includes an annular electrode surrounding the effective display region; a cathode is electrically connected with the annular electrode; the virtual region is located at an outer side of the annular electrode away from the effective display region.