Provided are a light-emitting device, a manufacturing method thereof and an electronic apparatus including the same, the light-emitting device including: a first electrode; a second electrode facing the first electrode; a middle region including an emission layer between the first electrode and the second electrode and an electron transport region between the second electrode and the emission layer; and an anti-oxidation layer between the second electrode and the electron transport region. The electron transport region includes an inorganic electron transport layer that comprises a metal oxide layer including a metal oxide.