The present invention relates to a process for preparing an epidermal growth factor-like peptide (EGF-like peptide) comprising the following amino acid sequence, C1(X)7C2(X)4-5C3(X)10-13C4(X)C5(X)8C6 [SEQ NO: 49] wherein C1-C6 are each cysteine and each X is independently a natural or unnatural amino acid, and wherein said EGF-like peptide has three intramolecular disulfide bonds; said process comprising the steps of: (I) preparing a first peptide fragment wherein the N-terminal amino acid is protected by a protecting group PG1, which is selected from Boo and Fmoc; (II) preparing a second peptide fragment wherein the C-terminal amino acid is protected by a protecting group PG2, which is selected from trityl, chlorotrityl and t-butyl; and wherein the amino acid side chains in said first and second peptide fragments are optionally protected; (III) coupling the C-terminal amino acid of said first peptide fragment with the N-terminal amino acid of said second peptide fragment in solution to form a linear protected EGF-like peptide; (IV)(a)(i) treating the linear protected EGF-like peptide formed in step (III) with iodine to form an oxidized mixture; (ii) globally deprotecting the oxidized mixture obtained in step (IV)(a)(i) by treating with trifluoroacetic acid (TFA); (iii) treating the deprotected oxidized mixture obtained in step (IV)(a)(ii) with DMSO/DTT to form a cmde EGF-like peptide; or (IV)(b)(i) globally deprotecting the linear protected EGF-like peptide obtained in step (III) by treating with trifluoroacetic acid (TFA); (ii) treating the deprotected mixture obtained in step (IV)(b)(i) with DMSO to form a cmde EGF-like peptide; and (V) optionally purifying the crude EGF-like peptide. Further aspects of the invention relate to processes for preparing EGF-like peptides using various fragment condensations.