Provided are a microdevice substrate, a display panel, a preparation method, and a display apparatus. The microdevice substrate includes a base substrate, multiple microdevices disposed on one side of the base substrate, and support structures. The support structures are connected to the multiple microdevices. A support structure includes a first support portion and a second support portion. The first support portion is fixedly connected to the base substrate. The second support portion is connected between a microdevice and the first support portion. The microdevice and the second support portion are suspended on the same side of the base substrate. A guide structure is disposed on the second support portion. The guide structure affects a broken position of the second support portion after being stressed.