A display substrate and a display device are provided. The display substrate includes: a base substrate; a plurality of pixel units arranged on the base substrate, the plurality of pixel units are arranged in an array in a first direction and a second direction, each pixel unit includes a plurality of sub-pixels, each sub-pixel includes a light emitting element and a pixel driving circuit used to drive the light emitting element, and the pixel driving circuit includes a first transistor; and a plurality of gate lines arranged on the base substrate, the plurality of gate lines include a plurality of first gate lines used to provide a scanning signal to gate electrodes of the first transistors of the pixel driving circuits in a plurality of rows of sub-pixels respectively. At least one first gate line includes a first gate sub-line, a second gate sub-line and a plurality of connecting lines.