Provided are a display substrate, a method for manufacturing the display substrate, and a display device. The display substrate has a display area, an opening area being provided in the display area, and the display substrate includes: a base substrate; at least one isolation structure provided on the base substrate and surrounding the opening area, and the isolation structure includes a first sidewall; a first filling structure provided on a side of the first sidewall away from the opening area, the first filling structure includes a second sidewall facing the first sidewall and a third sidewall facing away from the first sidewall; a first inorganic thin film disposed between the isolation structure and the filling structure; and a second inorganic thin film covering the isolation structure and the first filling structure.