A display substrate includes a light-emitting structure layer provided on a base; the light-emitting structure layer includes an anode layer, a first film layer group, and a second film layer group that are sequentially stacked; the anode layer includes one or more anodes; the first film layer group includes at least one film layer located between an anode and a cathode in an OLED device; the second film layer group includes a cathode, and at least one film layer located between the anode and the cathode in the OLED device; the orthographic projection of the second film layer group on the base includes the orthographic projection of the first film layer group on the base, an overlapping portion of all film layers in the first film layer group serves as a first portion of the first film layer group.