A display substrate has a central region and an edge region, and includes a plurality of structural units arranged in an array in the central region and the edge region; each structural unit is provided with a plurality of through holes, and divided by the plurality of through holes into a pixel island for displaying and a bridge portion for transmitting signals; the plurality of through holes are arranged around the pixel island; a part of the bridge portion is positioned between the pixel island and the through holes, and another part of the bridge portion is positioned between adjacent through holes; and a pixel pitch between adjacent pixel islands in the structural units in the edge region is equal to a pixel pitch between adjacent pixel islands in the structural units in the central region.