A backlight driving method and a display panel are provided. The backlight driving method provided by the present application uses pulse width modulation technology to modulate a low-level pulse width of a scan signal. By increasing a low-level pulse width to increase the pulse period, a number of high-level pulses is reduced, thereby reducing a high-level pulse time. Therefore, a time for a TFT to receive a high-level voltage is also reduced, thereby improving a stability of the TFT and also improving a problem of a threshold voltage drift of the TFT.