Provided is a display substrate. The display substrate includes: a display region and a bonding region on a side of the display region, wherein the display region includes a plurality of pixel columns; and a wiring structure, disposed between the display region and the bonding region and including a plurality of traces, wherein one of the plurality of traces corresponds to one of the plurality of pixel columns, the plurality of traces are electrically connected to the plurality of pixel columns to supply an electric signal to pixels, and each of the plurality of traces includes a plurality of sub-traces, wherein at least one of line lengths and line widths of sub-traces corresponding to at least a part of the plurality of traces are different, such that total resistances of the at least the part of the plurality of traces are basically equal.