A display substrate, including: a base substrate. The base substrate includes a display region, and the display region is provided with a plurality of sub-pixels and a conductive protective structure. At least one sub-pixel among the plurality of sub-pixels includes: a light-emitting element and a driving circuit that drives the light-emitting element to emit light. The light-emitting elements and the conductive protective structure are located on the side of the driving circuit away from the base substrate. The conductive protective structure includes at least one conductive part, and the at least one conductive part is located at an interval between respective light-emitting parts of light-emitting elements of at least two adjacent sub-pixels. The conductive protective structure is electrically connected to a signal terminal, and is configured to reduce carrier transmission between adjacent sub-pixels.