A display substrate and a display device are provided. The display substrate includes: a base substrate and a plurality of sub-pixels arranged on the base substrate, the plurality of the sub-pixels are arranged in an array, the sub-pixel includes: a reference signal line and a second scan line; and a sub-pixel driving circuit including a driving transistor and a second transistor, a gate electrode of the second transistor is coupled to the second scan line, a first electrode of the second transistor and the reference signal line form an integrated structure, a second electrode of the second transistor is coupled to a gate electrode of the driving transistor.