A display substrate and a preparation method therefor, and a display apparatus are provided. The display substrate includes a display area and a non-display area surrounding the display area, the display area including a first display region, a second display region and a fan-out wiring region, the second display region being located between the first display region and the fan-out wiring region; the first display region including a plurality of first sub-pixels, the first sub-pixel including a first pixel circuit and a first light emitting element; the second display region including a plurality of second sub-pixels, the second sub-pixel including a second pixel circuit and a second light emitting element; the fan-out wiring region including a plurality of data fan-out lines and a plurality of third sub-pixels, the third sub-pixel including a third light emitting element.