A method and system for correcting a difference in contrast agent density in a sequence of contrast-enhanced image frames. A reference image frame is defined in the sequence of contrast-enhanced image frames, and segmentation is performed on the reference image frame to determine a location of a region of interest within the reference image frame. The region of interest is a region of the reference image frame that contains contrast agent. Other image frames in the sequence of contrast-enhanced images are corrected based on a difference in contrast agent density/image intensity in the region of interest relative to the region of interest in the reference image frame.