Provided is a microfluidic chip. The microfluidic chip includes a first substrate and a second substrate disposed opposite to each other and drive electrodes, first sensing electrodes and second sensing electrodes disposed on a side of the first substrate. A microfluidic channel is formed between the first substrate and the second substrate and configured to accommodate at least one droplet. Different drive voltage signals are applied to adjacent drive electrodes, so as to drive the at least one droplet to move. Detection signals are applied to the first sensing electrodes and the second sensing electrodes, and a position of the at least one droplet is determined according to a change in capacitance between one first sensing electrode and an electrode corresponding thereto and a change in capacitance between one second sensing electrode and an electrode corresponding thereto when the at least one droplet flows by.