Disclosed herein are a non-elutable antibacterial glass composition and a method for preparing an antibacterial glass power using same, wherein the non-elutable antibacterial glass composition contains ingredients harmless to the human body, and has high durability and high water resistance, and thus can maintain an antibacterial function semipermanently. In addition, the present invention relates to a novel silicate-based antimicrobial glass composition which contains SiO2, which is a glass forming agent, as the main ingredient, and to which the addition of P2O5 was intentionally precluded.