An array substrate and a display device are provided. The array substrate includes a plurality of sub-pixel groups, each sub-pixel group includes two first color sub-pixels, one second color sub-pixel and one third color sub-pixel; in each sub-pixel group, a first connection line between centers of the two first color sub-pixels intersects with a second connection line between a center of the second color sub-pixel and a center of the third color sub-pixel, and a length of an orthographic projection of a third connection line, between a center of one of the first color sub-pixels and the center of the second color sub-pixel, on the second connection line is different from a length of an orthographic projection of a fourth connection line, between the center of the one of the first color sub-pixels and the center of the third color sub-pixel, on the second connection line.