A production line assembly for laser wall breaking of plant spores includes a material conveying module, a laser wall breaking module, and a stock solution recycling module which are successively connected by means of pipelines. The material conveying module is used for conveying a suspension liquid to be subjected to laser wall breaking to the laser wall breaking module; the laser wall breaking module is formed by a plurality of light energy receiving regions being successively connected along the conveying direction of the suspension liquid; each light energy receiving region comprises a laser, a light energy receiver, and an XY limiter; the suspension is circulated in the light energy receivers, and the lasers are in one-to-one correspondence with the light energy receivers and are used for performing laser wall breaking on the suspension liquid; the lasers are used for irradiating the light energy receivers horizontally or from top to bottom; and the light energy receivers are mounted on the XY limiters, and the XY limiters are used for adjusting the positions of the light energy receivers in X- axis and Y- axis directions.