Guidance may be provided to users, at least in part, by graphic overlays on displays of ultrasound images. The guidance may assist users in acquiring images suitable for making hepato-renal index (HRI) measurements. In some examples, the guidance may assist the user in finding the proper imaging plane including a portion of the liver and kidney. In some examples, the guidance may further assist the user in proper placement of regions of interest within the image for calculating the HRI, such as at the same depth and appropriate portions of the liver and kidney. In some examples, one or more processors may analyze the image to ensure the quality is suitable for calculating the HRI.