A method for porating cells for loading with a substance, preferably nuclides for radiotherapy, performs porating in a porating device with cells to be porated in a liquid with the substance. The cell membranes are permeabilized by sonoporation or laser poration, then the cells are incubated in a porating device incubation section. Moreover, a device for porating cells, in particular by sonoporation or laser porating, and for loading cells, particularly immunocompetent cells, in particular γδ T cells, includes inlet and outlet openings for a cell solution with cells to be porated and a substance to be taken up by the cells as a load and preferably microbubbles. A poration arrangement extending between the openings has a collector at the inlet opening for collecting cell solution and a reactor in flow connection with the collector. Inside the reactor the cell membranes may be porated and the cells may uptake the load.