Imaging devices, systems, and methods for determining parameters of an object based on chemical indicators detected during a decode event are described herein. An example device includes: a housing; one or more processors; an imaging assembly at least partially disposed within the housing, the imaging assembly including: an imaging sensor operable to capture an image of an object in a FOV; and a computer-readable media storing machine readable instructions that, when executed, cause the one or more processors to: after capturing the image of the object in the FOV, determine whether a decode indicia is present in the image of the object; initiate a decode event for the decode indicia present in the image of the object; detect a chemical indicator in proximity to the decode indicia in the image of the object; and determine, based on the chemical indicator, one or more parameters associated with the object.