- 专利标题: UNIVERSAL LINKER REAGENTS FOR DNA SYNTHESIS
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申请号: US18551834申请日: 2022-03-22
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公开(公告)号: US20240209018A1公开(公告)日: 2024-06-27
- 发明人: Michael W. REED , Cheng-Hsien WU , John COOPER , Robert O. DEMPCY
- 申请人: GENSCRIPT USA INC. , CustomArray, Inc.
- 申请人地址: US NJ Piscataway
- 专利权人: GENSCRIPT USA INC.,CustomArray, Inc.
- 当前专利权人: GENSCRIPT USA INC.,CustomArray, Inc.
- 当前专利权人地址: US NJ Piscataway
- 国际申请: PCT/US2022/021287 2022.03.22
- 进入国家日期: 2023-09-21
- 主分类号: C07H21/04
- IPC分类号: C07H21/04 ; C07C247/04 ; C07C275/10 ; C07D491/18 ; C07F9/24 ; C25B3/05 ; C25B3/25 ; C25B3/29
摘要:
Provided herein are methods and compositions for oligonucleotide synthesis utilizing universal linker phosphoramidites. Methods and reagents are described with DNA synthesis using controlled pore glass (CPG) solid supports, and on platinum coated electrodes for electrochemical DNA synthesis. The universal linkers can be used as spacers in single-column PCR primer synthesis to generate 2 strands with free 3′-hydroxy termini after cleavage. The methods and compositions utilize a solid support system for synthesis of oligonucleotides, wherein the support has platinum electrodes and a universal linker, optionally wherein the platinum electrode is coated with an amine. The methods and compositions further describe use of universal linker phosphoramidites and the platinum electrode is coated with a monosaccharide, or a disaccharide.
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