A method and a circuit for driving a display panel, and a display device are disclosed. The display panel includes rows of sub-pixels. The method includes a display process, which includes: generating an initial driving signal for each row of sub-pixels according to image information of an image to be displayed; adjusting the initial driving signal for each row of sub-pixels according to a compensation gain of each row of sub-pixels, so as to obtain a target driving signal, the compensation gain of each row of sub-pixels is an actual brightness coefficient of the row of sub-pixels with respect to a reference row of sub-pixels during the display panel displaying an image with a test gray scale; and driving each row of sub-pixels to display according to the target driving signal for the row of sub-pixels, so as to enable each row of sub-pixels to reach target brightness.