A display substrate has a first display region and a second display region, and includes first light-emitting device groups disposed in the first display region, first pixel circuit groups disposed in the second display region, lead groups, and a plurality of second pixel circuits disposed in the second display region. A first light-emitting device group includes N first light-emitting devices. A first pixel circuit group includes N first pixel circuits. A lead group includes N leads, an i-th first light-emitting device is electrically connected to an i-th first pixel circuit through an i-th lead, and lengths of 1st to N-th leads increase. At least one line of second pixel circuits is disposed between the 1st first light-emitting device and the 1st first pixel circuit. The N leads extend from the first display region to the second display region passing through the at least one line of second pixel circuits.