A display substrate and a display device. The display substrate includes a driving substrate, an anode layer, a pixel definition layer, a light emitting functional layer, a cathode layer and an encapsulation layer; the driving circuit includes at least one transistor, the at least one transistor includes a semiconductor layer, the anode layer includes a plurality of anodes, the display substrate includes a plurality of pixel openings; the plurality of pixel openings are configured to at least overlap with the plurality of anodes; each of the anodes includes a main body portion and a protrusion portion, the protrusion portion is connected with the main body portion; the light emitting functional layer includes a plurality of light emitting portions which are arranged in contact with the main body portions of the plurality of anodes.