A display substrate includes a base substrate, a pixel circuit layer, a first planarization layer, at least one transparent conductive layer, and a plurality of first light-emitting elements. The base substrate includes a first display region and a second display region, wherein the first display region at least partially surrounds the second display region. The pixel circuit layer is located in the first display region, and includes a plurality of pixel circuits, wherein the plurality of pixel circuits include a plurality of first pixel circuits. A plurality of first light-emitting elements are located in the second display region. The first planarization layer is located on a side of the pixel circuit layer away from the base substrate, and is located in the first display region and the second display region. The transparent conductive layer is located on a side of the first planarization layer away from the base substrate and includes a plurality of first transparent conductive lines and at least one auxiliary structure. The plurality of first light-emitting elements and the plurality of first pixel circuits are coupled through the plurality of first transparent conductive lines. An orthographic projection of the auxiliary structure on the base substrate is overlapped with an orthographic projection of at least one of the pixel circuits on the base substrate.