A display substrate has a display region including a functional region and a main display region; the functional region has at least one functional unit, each of which has a blank region, a winding region and a circuit region; the display substrate includes a base substrate, and plural conductive layers on the base substrate; the plural conductive layers form at least one hollow pattern, each of which is in the blank region; the conductive layers include a first signal line, which includes first, second and third line segments in the main display region, the circuit region and the winding region, respectively; extending directions of the first and second line segments are the same, the third line segment electrically connects the first and second line segments together, and the third line segment includes at least two line sub-segments with different extending directions.