Disclosed are a display substrate and a preparation method therefor, and a display apparatus. The display substrate includes a display region and a bonding region, wherein the display region includes M pixel rows arranged sequentially, at least one pixel row includes a scan signal line and a plurality of sub-pixels arranged sequentially along an extension direction of the scan signal line, at least one sub-pixel includes a pixel drive circuit connected with the scan signal line, the pixel drive circuit at least includes a storage capacitor and a first transistor as a first initialization transistor, and the scan signal line at least includes a second scan signal line that controls the first transistor to be turned on or off, and in the at least one pixel row, the second scan signal line is arranged on a side of the storage capacitor close to a display region boundary.