A manufacturing method of a touch substrate, a touch substrate, and a touch displaying device. The touch substrate includes a base plate; touch electrodes; and at least three light adjustment layers stacked on a side of the base plate, and adjacent two of the light adjustment layers have different refractive indexes; among the at least three light adjustment layers, a thickness of a light adjustment layer close to the base plate is greater than the thicknesses of another light adjustment layer adjacent to the light adjustment layer; the touch electrodes include a first touch electrode and a second touch electrode that are disposed on a side, backing onto the light adjustment layers, of the base plate, the first touch electrode and the second touch electrode are disposed in different layers, and the second touch electrode is disposed on a side, backing onto the base plate, of the first touch electrode.