A display substrate includes a substrate, a reflective layer, a plurality of first signal lines, and an active layer that are stacked in sequence. The active layer includes a plurality of active pairs, and an active pair includes two active patterns. An orthographic projection of an active pattern on the substrate overlaps with an orthographic projection of a first signal line on the substrate, and a portion of the active pair that does not overlap with the first signal line has a gap. An orthographic projection of the reflective layer on the substrate covers at least part of a gap of at least one active pair.