Disclosed are a display substrate, a display substrate motherboard and a display apparatus. The display substrate includes a display region and a bonding region located on a side of the display region, wherein the bonding region includes a fanout region, the fanout region includes a first anti-static area and a first wiring area located around the first anti-static area, the first wiring area includes a plurality of fanout wires, the first anti-static area includes at least one electrostatic protection structure disposed between the plurality of fanout wires, and the electrostatic protection structure includes at least one pair of electrostatic protection lines, and the pair of electrostatic protection lines includes two electrostatic protection lines disposed symmetrically about a center line extending in a first direction.