A display substrate includes pixel circuits and light-emitting devices. The pixel circuits include first pixel circuits and second pixel circuits. The light-emitting devices include first light-emitting devices and second light-emitting devices. A first pixel circuit is coupled to a first light-emitting device, and the first pixel circuit is at least partially opposite to the first light-emitting device. A second pixel circuit is coupled to a second light-emitting device, and an orthographic projection of the second pixel circuit and an orthographic projection of the second light-emitting device have no overlap. A width-to-length ratio of a channel of a driving transistor in the first pixel circuit is greater than a width-to-length ratio of a channel of a driving transistor in the second pixel circuit; and/or a channel capacitance of a compensation transistor in the first pixel circuit is larger than a channel capacitance of a compensation transistor in the second pixel circuit.