Display substrate and display device are provided. The display substrate includes: a base and sub-pixels thereon, the sub-pixel includes a light-emitting element and a sub-pixel driving circuit including a driving transistor and a compensation transistor; the compensation transistor has a first electrode coupled to a second electrode of the driving transistor, and a second electrode coupled to a gate electrode of the driving transistor; the compensation transistor includes a gate electrode and an active layer including a channel portion; the display substrate includes a pixel definition layer and shielding patterns, at least a portion of the shielding pattern is located between the pixel definition layer and the gate electrode of the compensation transistor, and an orthographic projection of the shielding pattern onto the base at least partially overlaps with an orthographic projection of corresponding channel portion of the compensation transistor onto the base.