A display substrate is provided. The display substrate includes a pixel defining layer having a plurality of apertures corresponding to the sub pixels of at least two different colors. Orthographic projections of the plurality of apertures on the base substrate each are divided by the orthographic projections of the plurality of first power supply lines on the base substrate into a first portion and a second portion. For the sub pixels of the at least two different colors, a first area ratio is a ratio between areas of the first portions of the orthographic projections of the apertures on the base substrate, a second area ratio is a ratio between areas of the second portions of the orthographic projections of the apertures on the base substrate, and a ratio between the first area ratio and the second area ratio is in a range from 0.8 to 1.2.