The disclosure provides a display panel, a manufacturing method thereof and a display device, relating to a field of display technology. The display panel includes a base substrate, a driving layer and a light-emitting layer. The light-emitting units formed by the light-emitting layer includes a first electrode layer, a first insulating layer, a control electrode layer, a second insulating layer, an active layer, a second electrode layer, and an organic light-emitting layer sequentially distributed. In the embodiments of the present disclosure, the second insulating layer is configured to separate the control electrode layer from the organic light-emitting layer and the active layer.