Provided in the present invention are a time calibration method and device, and a readable storage medium. In the time calibration method, a first data frame comprises a plurality of first code blocks, and a second data frame comprises a plurality of second code blocks, and therefore the first data frame and the second data frame can be used as a multiple frame comprising the plurality of code blocks, so as to be adapted to an Ethernet interface, which is divided into a plurality of sub-slots. In addition, according to an acquired first moment value, second moment value, third moment value and fourth moment value, the time offset of a device to be synchronized compared with a reference device can be known.