The present invention provides a photosensitive material for use in electrophotography which is prepared by forming a barrier layer containing a peptide polymer between a conductive support and a photosensitive layer. This photosensitive material impedes unnecessary charge infiltration through the conductive support, maintains an appropriate charge acceptability, imparts an adhesive property of the photosensitive layer in relation to the support or flexibility of the photosensitive material, and prevents deterioration of such characteristics as photosensitivity, residual potential, etc. When some acceptor or donor is additionally mixed in said barrier layer, the electrification property thereof is improved.