发明授权
- 专利标题: Method for preparation of a recombinant DNA phage
- 专利标题(中): 重组DNA噬菌体的制备方法
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申请号: US87167申请日: 1979-10-22
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公开(公告)号: US4348478A公开(公告)日: 1982-09-07
- 发明人: Eiichi Nakano , Tsutomu Masuda , Narimasa Saito , Danji Fukushima
- 申请人: Eiichi Nakano , Tsutomu Masuda , Narimasa Saito , Danji Fukushima
- 申请人地址: JPX Noda
- 专利权人: Noda Institute for Scientific Research
- 当前专利权人: Noda Institute for Scientific Research
- 当前专利权人地址: JPX Noda
- 优先权: JPX53-130437 19781025
- 主分类号: C12N15/09
- IPC分类号: C12N15/09 ; C12N15/00 ; C12P19/34 ; C12R1/91
摘要:
A method for the preparation of a novel recombinant DNA, which comprises (1) cleaving with an endonuclease a temperate phage DNA having an endonuclease-sensitive region not in the DNA segment participating in the replication of phage DNA and the integration of phage DNA into a host chromosome but at least in the DNA segment carrying genetic information for the coat protein production and another DNA carrying intended genetic information, (2) adding DNA-ligase to the mixture of both cleft DNA's, and (3) recovering from the mixture a phage DNA having its coat protein producing ability deleted by the replacement of the DNA segments carrying genetic information for the coat protein production with a DNA fragment carrying the intended information.
公开/授权文献
- US5376943A Moving vehicle transponder 公开/授权日:1994-12-27