In an electrophotographic system in which latent images of negative and positive polarities formed electrostatically on a photoconductive member or a dielectric member are successively developed with two different kinds of toners and then transferred onto a transfer medium, an image transfer and sheet separation apparatus comprises a precharger for uniformalizing the different polarities of the toners carried on the photoconductive member or the dielectric member, a transfer charger for transferring the toners onto the transfer medium, and a conductive member for image transfer and sheet separation located to become intimately engaged by the transfer medium and provided with means for applying a voltage.